Clonatest Lipase BR 1×36 mL Per Box
Lipase BR 1 × 36 mL - Clonatest
The method for the determination of lipase is based on the cleavage of specific chromogenic lipase substrate 1,2-O-dilaurylrac-glycero-3-glutaric acid-(6’methyl-resorufin)-ester emulsified in stabilized micro-particles. In the presence of specific activators of pancreatic lipase as colipase, calcium ions and bile acids, the substrate is con-verted to 1,2-O-dilauryl-racglycerol and glutaric acid-6’-methylresorufinester which decomposes spontaneously to glutaric acid and methylresorufin. The increase of absorbance at 580 nm, due to methylresorufin formation, is proportional to the activity of lipase in the sample.
REAGENT
R1. Tris buffer, pH 8.3 40 mmol/L, Colipase ≥ 1 mg/L, Desoxycholate ≥ 1.8 mM/L, Taurodesoxycholate ≥ 7.0 mM/L. R2. Tartrate buffer, pH 4.0 15 mM/L, Lipase Substrate ≥ 0.7 mM/L, Calcium Ions ≥ 1 mM/