Clonatest AST-GOT BR opt 5×40 mL Per Box

AST-GOT BR opt 5×40 mL - Clonatest

Aspartate aminotransferase (AST/GOT) catalyzes the transfer of the amino group from aspartate to oxoglutarate with the formation of glutamate and oxalacetate. The latter is reduced to malate by malate dehydrogenase (MDH) in the presence of reduced nicotinamide adenine dinucleotide (NADH)

. The reaction is monitored kinetically at 340 nm by the rate of decrease in absorbance resulting from the oxidation of NADH to NAD , proportional to the activity of AST present in the sample.

L-Aspartate 2-Oxoglutarate L-Glutamate Oxalacetate Oxalacetate NADH H L- Malate NAD This test has been formulated according the standarized method described by IFCC.

REAGENTS

R1. AST substrate. TRIS buffer 121 mmol/L pH 7.8, L-aspartate 362 mmol/L, malate dehydrogenase> 460 U/L, lactate dehydrogenase> 600 U/L. R2. AST coenzyme. NADH 1.3 mmol/L, 2-oxoglutarate 75 mmol/L. Biocides.