Cromatest LDL-Cholesterol Direct 40 mL Per Box

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LDL-Cholesterol Direct 40 mL - Cromatest

PRINCIPLE

This direct method for quantifying cholesterol in low-density lipoproteins (LDL) is an homogeneous enzymatic test in which the differential precipitation and further sedimentation of the rest of lipoproteins and quilomicrons is avoided. The procedure comprises two steps. In the first step cholesterol in lipoproteins other than LDL in the test sample are decomposed by the simoultaneaus action of cholesterol esterase (CE) and cholesterol oxidase (CO) at pH 7.0, giving as end products cholestenone and hydrogen peroxide, the latter being decomposed to water and oxygen by catalase. In the second step a surfactant which specifically acts on LDL is added to the reaction product of the first step being the remaining cholesterol quantified by a Trinder´s type reaction in which the aniline derivate, HDAOS*, and 4-aminoantipyrine (4-AA) as a coupling reagent are condensed by the H2O2 in presence of peroxidase (POD) to form a red quinoneimine dye proportional to the concentration of LDL-cholesterol present in the sample. * N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline

Cromatest LDL-Cholesterol Direct 40 mL Per Box

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