Cromatest Cholesterol MR 2×50 mL Per Box

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Cholesterol MR 2 × 50 mL - Cromatest

This method for the measurement of total cholesterol1,2 in serum involves the use of three enzymes: cholesterol esterase (CE), cholesterol oxidase (CO) and peroxidase (POD). In the presence of the former the mixture of phenol and 4-aminoantipyrine (4-AA) are condensed by hydrogen peroxide to form a quinoneimine dye proportional to the concentration of cholesterol in the sample.

REAGENT COMPOSITION

R1 Monoreagent. PIPES 200 mmol/L pH 7.0, sodium cholate 1 mmol/L, cholesterol esterase  250 U/L, cholesterol oxidase  250 U/L, peroxidase  1 KU/L, 4-aminoantipyrine 0.33 mmol/L, phenol 4 mmol/L, non-ionic tensioactives 2 g/L (w/v). Biocides. CAL Cholesterol standard. Cholesterol 200 mg/dL (5.18 mmol/L). Organic matrix based primary standard. Concentration value is traceable to Standard Reference Material 909b

Cromatest Cholesterol MR 2×50 mL Per Box

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